I am new to the world of PRP subcutaneous injection. Was recently introduced by a sales rep for PRP tubes. Would some experienced PRP injectors give me some comments about my protocol?
1. Draw 8-10mL of Venous blood from Cephalic vein
2. Using the centrifuge to spin for 30 minute until 3 layers of precipitate appear.
3. Using a 23G needle syringe to draw up approximately 2.5mL to 3mL of PRP (Platelate Rich Plasma) from the middle layer of precipitate.
4. Immediately inject to the desired area using a 27G needle syringe.

The idea behind PRP injection is not instant filling effect but to improve collagenesis in the desired area.
Can someone share their experience with PRP injection and how are the results compared to Sculptra and Restylane Vital?

HI Kenneth,

We have had some good results with PRP, use it for acne scar rejuvenation, uptake of grafts on chronic ulcers, for follicular grafts both in vitiligo and hair transplant surgeries... we haven't used it as a filler but intra-dermal injections like mesotherapy for facial rejuvenation too

Dr Kenneth

Are you from Taiwan or Hong Kong?

hi Dr Dolloway.

I practice in Taipei, Taiwan just minutes away from the Taipei 101 building. PRP is becoming quite popular in Asia. A lot of docs are using this in a mesotherapy-like procedure. Orthopods have been using PRP knee injection in conjunction to Hyaluronic acid in osteoarthritis patients. Not sure if the results are placebo related or not. Many patients swear by PRP injection of the knee joints.

Can anyone please share their protocol for creating PRP with a regular centrifuge and syringes?

The protocol for preparation and use of the PRP is very simple. The important point is to use a container class 2a and the price of container depends on the concentration of platelets and the final result.

I do know if you are to use a regular centrifuge that requires tubes (vacutainers), often done in Europe and Asia, you will have to spin the blood 3 times at different rpm's to get the proper concentration of platelets. This is the reason that many people often complain the results are minimal to none. Concentration is important..at least 1.5 mil -2mil....any more that 2mil creates inhibition as demonstrated by numerous studies Guisti and Kevy both. And then your yield is only about 3-5cc of product. Sometimes it is better to spend more up front and profit in time saving and yielding more finished product 10cc. Harvest is the gold standard. Sorry, just speaking from experience of the last 4 1/2 years doing this.

Thanks for your input. The biggest concern I can see is that proper technique would be critical to ensure consistent concentrations of the PRP.

Hi S Parker,

Thank you for your post. I am currently looking into a few different systems for our clinic. I have look at Harvest as you have suggested. Are you able to comment between the Harvest System and the Regen system at all? I am from Australia and it seems that Harvest really do not have much of a presence here as yet.

Thanks

Kim

We have had good experiences and great results with Selphyl.

I am using regen lab kit with autologous thrombine platelet activation. It gives a thick gel that you can use superficially - especially in the peri orbital region - with constant success. Of course in the zygomatic area you can create a scaffold with HA and "build up" with the gel.

There are multiple proprietary systems out on the market and some of which are all-in-one "black box" devices that take up a lot of space (and cost a lot of money!). I have been using Regenlab tubes for over 4 years and it is still the simplest system to consistently obtain high quality PRP. All you need are the tubes if you already have a centrifuge. Selphyl is similar. The bench space is essentially taken up by the centrifuge. Others in Australia have tried normal coag tubes but it's exceedingly difficult to obtain the platelets from the buffy coat in a consistent manner without contamination by the white and red cells. It's fiddly and your results will be inconsistent. I acknowledge that Regenlab tubes do yield roughly 50% of the white cells. As for using plain 10ml syringes, you would need to add citrate and have a method of sealing the syringe while spinning. This gives you a home made system of "coag tubes" but you are still left with the issue of extracting the PRP (though perhaps not potentially as fiddly). I suggest you try the Regenlab tubes for yourself. They do sell various kits for different indications but we can buy the tubes packaged separate from a kit here.